RNA modification services
Four route lines for reading a modification: single-base sequencing for position and level, RIP-sequencing for transcript-level enrichment, modification arrays for multiplexed screening, and a dedicated GlycoRNA line.
01The four route lines
Arraystar RNA modification services are organized by the resolution the question needs. Each line answers a different kind of question, and the lines are designed to be combined: screen first, then resolve the sites that matter.
Single-Base RNA Modification Sequencing
Sixteen chemical and enzymatic workflows that report modification type, position and level site by site.
Site-levelposition & level GROUP_02RNA Modification RIP-Sequencing
Nine antibody-based enrichment workflows, from m6A MeRIP-Seq to Ψ-RIP-Seq and hMeRIP-Seq.
Transcriptenrichment GROUP_03GlycoRNA Solution
An integrated line for glycosylated RNA: array screening, sequencing of glycosylated small RNA, and blotting for direct detection of the glycoRNA population.
Dedicatedglyco line GROUP_04RNA Modification Arrays
Array formats that screen modification levels across many transcripts in parallel.
Multiplexedscreening02Choosing between the lines
You need the exact site
Single-base sequencing reports where a modification sits and how much of it is present, which is what mechanism studies and site-level comparisons are built on.
You need many samples or many transcripts
RIP-sequencing and arrays trade site-level resolution for throughput: they report modification profiles across transcripts or across sample sets, and point to where site-level work is worth doing.
You need an independent readout
LC-MS on isolated RNA measures nucleoside content directly, independently of any sequencing or array workflow, and is useful when a level change needs to be confirmed by a second method.
03How a project runs
- Define the question. The modification of interest, the sample type and the comparison being made are discussed before a route is fixed.
- Check the sample. Input type and quality are reviewed against what the chosen workflow needs.
- Run the workflow. Sample QC, the modification-specific treatment or enrichment, library construction and sequencing.
- Analyze and report. Site calling or profile quantification, followed by the analysis set and tables described on each service page.
| Need | Recommended line | Request Quote |
|---|---|---|
| Exact position and level of a modification | Single-base sequencing | |
| Modification profile across transcripts | RNA Modification RIP-Sequencing | |
| Screen many targets or many samples | RNA Modification Arrays | |
| Glycosylated RNA | GlycoRNA Solution | |
| Independent quantification | LC-MS on isolated RNA |
04FAQ
Which service line should I start from?
Start from the resolution the conclusion needs. If the study turns on a specific site, go straight to single-base sequencing. If the goal is to compare conditions or samples broadly, screening first and then resolving the interesting sites is usually the shorter route.
Can the lines be combined in one project?
Yes, and that is how most projects are set up: a screening step narrows the field, and site-level or LC-MS work confirms what the screen flagged.
What sample types are accepted?
Total RNA, cells, tissue or blood. The exact preparation notes are listed on each service page.
How do I get a price?
Send the modification of interest, the sample type and the approximate number of samples through the request form, and the scope will be confirmed before a quote is issued.