Small RNA Modification Array (m6A / O8G / m7G / m5C / m1A / Ψ)
Keep the comparison inside the small RNA pool: modification-related signals on small RNA targets, with several marks on one panel when length class is the first constraint.
01What the service reports
Small RNA Modification Array scores modification-related signal on small RNA panel targets, including readouts associated with m6A, O8G, m7G, m5C, m1A and Ψ. The comparison is across samples on that small RNA panel.
Small RNA is not a shortened mRNA list. This array keeps the experiment in the small RNA length class so that miRNA-scale and other short targets are not forced through a long-RNA design.
The output is panel signal for those short targets. Identity and abundance of small RNA species still belong to small RNA sequencing; single-base chemistry still belongs to site-level services.
02Benefits
- Right length class: the panel is built for small RNA targets, not scaled down from mRNA arrays.
- Several marks on short RNA: related modification signals can be seen in one small RNA run.
- Condition contrasts: compare treatments or stages on the same small RNA panel.
- Stays in the short-RNA class: the panel is read on small RNA targets, so a result is not diluted by long transcripts that share the same mark.
- Natural follow-up: shortlisted targets move to small RNA sequencing or to site-level chemistry.
| Service Name | RNA Class | Price |
|---|---|---|
| Small RNA Modification Array | Small RNA / total RNA |
03Background and use
When the question sits in the small RNA pool, a long-RNA array mixes length classes and blurs interpretation. A small RNA-specific panel answers whether modification-related signal on short targets changes between samples.
What the format gives and what it does not: the readout is comparative signal on panel members, so it ranks short-RNA targets without naming every species in the sample and without placing a mark at a nucleotide. Species identity belongs to small RNA sequencing, and position-level chemistry to the single-base services; the panel decides which targets are worth either.
04Workflow
How a small RNA panel screen is run:
- Prepare total RNA and check quality before hybridization.
- Hybridize the small RNA modification panel.
- Detect panel signals on the short-RNA targets.
- Normalize and compare across the sample set.
- Report which small RNA panel members carry a changing signal.
05Analysis and deliverables
Panel values are compared across samples so that small RNA targets with changing modification-related signal can be listed without leaving the short-RNA class.
Deliverables
- Small RNA panel signal table per sample
- Cross-sample comparison of short-RNA targets
- Follow-up notes for sequencing or site-level chemistry
- Structured project report
06Research applications
- Modification signal in the small RNA pool: whether short-RNA targets such as miRNA-scale species shift between conditions, treatments or stages.
- Small RNA against long RNA: whether a change seen on long transcripts also appears on short ones when both classes are read in the same study.
- Triage before deeper work: which short RNA targets are worth taking into small RNA sequencing or into site-level chemistry.
07Sample requirements
Total RNA is accepted; the assay is organized for the small RNA fraction within that input.
| Sample type | Notes |
|---|---|
| Total RNA | OD 260/280 ≥ 1.8 and RIN ≥ 7, with no visible degradation. Submit more than 5 µg total RNA (practical minimum 2 µg). |
| Cultured cells | Cell pellets are preferred. |
| Tissue | Fresh or frozen specimens; avoid necrotic material. |
08FAQ
Why not use an epitranscriptomic array for small RNA?
That panel is organized around longer transcripts and a different target set. A small RNA array keeps the comparison inside the short-RNA class when that is where the question sits.
Does this replace small RNA sequencing?
No. Sequencing names species and abundance in the small RNA pool. This array reports modification-related panel signal on short-RNA targets across samples.
Which marks are represented?
The panel covers several marks on small RNA targets, including m6A, O8G, m7G, m5C, m1A and Ψ related readouts.
Which research questions fit this readout?
Whether modification-related signal on small RNA panel targets changes with condition, before species-level or site-level follow-up is planned.
What should follow this array?
Small RNA sequencing when identity matters, or a single-base modification service when a position on a short RNA must be resolved.
09References
- Internal service overview: Small RNA Modification Array — multi-mark screening in the small RNA pool.