RNA Modification Arrays
Array formats that read modification levels across many transcripts at once, for screening work before site-level or quantitative confirmation.
01What this line is for
RNA modification arrays measure modification levels for a defined set of transcripts in parallel. They report how much modification is present on each target, which makes them a screening route rather than a mapping route.
Arrays are the practical answer when the question is “which of these many targets changed?” rather than “where exactly is the modification?”. Coverage is defined by the array design, and positions are not resolved, so an array result is normally followed by a single-base method on the targets that matter.
02The array services
Three array formats cover epitranscriptomic screening at different scopes.
Epitranscriptomic Array
A multi-modification array that reads modification levels across a defined transcript set.
Multi-markscreening ARRAY_02m6A Single-Nucleotide Array
An m6A-focused array for comparing modification levels across many targets.
Single-markscreening ARRAY_03Small RNA Modification Array
Modification levels on small RNA targets, covering miRNA, pre-miRNA, tRNA and tRF & tiRNA.
Small RNAscreening03GlycoRNA Solution
GlycoRNA Solution brings the glycosylated-RNA workflows together in one place: array screening, two sequencing routes that differ by RNA length, and blotting for direct detection of the glycoRNA population.
Glycosylation and chemical modification are read by different chemistries, so they are kept as a separate line rather than folded into the modification arrays.
GlycoRNA Array
Array-based screening of glycosylated RNA targets.
Screeningarray GLYCO_02GlycoRNA Sequencing (small RNA)
Sequencing of glycosylated small RNA: which short RNA species carry the signal.
Sequencingsmall RNA GLYCO_03GlycoRNA Blotting
Lectin-based detection of the glycoRNA population: overall abundance and molecular-size patterns for a sample.
Whole-classblot04Arrays inside a project
Narrow the field
Run the array across the sample set to see which targets change. This is the step that makes a large list into a short one.
Go to position level
Take the targets that moved into a single-base method, which reports the individual sites and their modification levels.
Add an independent readout
Where a level change carries the conclusion, LC-MS on isolated RNA measures nucleoside content independently of the array and the sequencing result.
05FAQ
Do arrays resolve single positions?
No. Arrays report modification levels for the transcripts on the array and do not resolve individual nucleotides. Position-level work is done with a single-base method.
When is an array the better first step?
When the study covers a large number of targets or samples and the first question is which ones changed. Screening first keeps the site-level work focused on the targets that matter.
How does the small RNA modification array differ from the epitranscriptomic array?
They differ in the molecules they cover. The small RNA array targets miRNA, pre-miRNA, tRNA and tRF & tiRNA, while the epitranscriptomic array covers a defined transcript set across several modification types.
Is the GlycoRNA line part of the modification arrays?
No. GlycoRNA Solution is its own line with array, sequencing and blotting options, listed above.
What sample types are accepted?
Total RNA, cells, tissue or blood. Preparation notes are given per service.