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Sample Submission Guide

Sample submission guide for RNA projects

Arraystar provides detailed sample submission guides for RNA projects, covering RNA sample amount and quality requirements, RNA sample shipment instructions, and biosample preparation protocols for RNA extraction.

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Sample Submission Guide for RNA Projects

Choose a guide below to review sample amount and quality requirements, shipment instructions, and biosample preparation protocols for your tRNA and tRF&tiRNA project.

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RNA Sample Submission

RNA amount requirements, RNA quality requirements, RNA sample shipment options, and the disclaimer for low sample amount or quality.

  • RNA Amount Requirements
  • RNA Quality Requirements
  • RNA Sample Shipment
  • Disclaimer for Low Sample Amount/Quality

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Biosamples for RNA Extraction

Biosafety guidance and recommended protocols for collecting and preparing cells, tissues, biofluids, and exosomes for RNA extraction.

  • Biosafety
  • RNA Extraction from Cells
  • RNA Extraction from Tissues
  • RNA Extraction from Biofluids or Exosomes
  • Biosample Shipment
  • Disclaimer for Low Sample Amount/Quality

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Need help with sample preparation?

If the recommended minimum sample amount is not obtainable, or if you have questions about biosample collection and shipment, contact our technical support by email at support@arraystar.com to make special arrangements.

Sample Submission FAQ

Common questions about RNA sample submission and biosample preparation

Where can I find the recommended RNA amount for my tRNA service?
The RNA Sample Submission guide lists recommended total RNA amounts per sample for tRNA-seq, tRF/tiRNA-seq, tRNA Charging Seq, tRNA modification sequencing, tRNA and tRF&tiRNA PCR arrays, and tRNA/mRNA modification LC-MS. Recommended minimums are intended for the entire experiment for a single attempt, including sample QC. Supplying twice the recommended minimum avoids project delays.
What should I do if my sample amount is below the recommended minimum?
If the recommended minimum amount is not obtainable, consult Arraystar technical support by email at support@arraystar.com to make special arrangements. In case of low sample amount or quality, we will contact you to decide whether to replace, proceed, or drop the sample from the study. Data quality and success rate may decrease with lowered sample amount or quality.
How should biosamples be prepared for RNA extraction?
Cells should be immediately lysed in TRIzol; RNAlater is not recommended for cells. For tissues, excise within 10 minutes, then use the RNAlater method, TRIzol method, fresh freezing in liquid nitrogen, or FFPE fixation. For biofluids, collect serum, plasma, or whole blood as directed, and isolate exosomes by your chosen method. Ship all biosamples on dry ice.

Ready to submit your RNA samples?

Contact our technical support team for sample amount and quality questions, or request a quote for your tRNA and tRF&tiRNA project.