Arraystar supplies pre-designed tRF&tiRNA-specific PCR primers for the accurate quantification of individual tRFs&tiRNAs in qPCR. All the primers have been validated in numerous sample types, and guarantee the most accurate and specific performance when used with Arraystar SYBR® Green qPCR Master Mix.
Validated qPCR primer pairs for individual tRF&tiRNA quantification
rtStar™ Pre-designed Human tRF&tiRNA Primer Sets are part of the nrStar™ tRF&tiRNA PCR System, and can be used in combination with the rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor) (AS-FS-003) for tRF&tiRNA detection and quantification.
All the primers have been validated in numerous sample types, and guarantee the most accurate and specific performance when used with Arraystar SYBR® Green qPCR Master Mix. Human and Mouse primer lists provide tRF&tiRNA names, sequences, source tRNAs, and catalog numbers.
Pre-designed tRF&tiRNA primer sets are validated qPCR primer pairs targeting individual tRFs and tiRNAs. Used with the rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor) and Arraystar SYBR® Green qPCR Master Mix, they enable accurate, specific quantification of individual tRFs&tiRNAs.
Standard catalog products — human and mouse versions available
| Product Name | Catalog No. | Size | Price |
|---|---|---|---|
| rtStar™ Pre-designed Human tRF&tiRNA qPCR Primer Pair | AS-NR-002-1-M | 200 reactions | |
| rtStar™ Pre-designed Mouse tRF qPCR Primer Pair | AS-NR-002M-1-M | 200 reactions |
Why researchers choose rtStar™ Pre-designed tRF&tiRNA Primer Sets
Reliable results with fully validated primers.
High sensitivity and specificity for individual tRF&tiRNA quantification.
Accurate quantification over a wide linear range.
Time and cost savings with validated primers and SYBR® Green assay.
All primers validated in numerous sample types.
Performs superbly with the rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor) (CAT# AS-FS-003) and Arraystar SYBR® Green qPCR Master Mix.
Primer validation and performance
The rtStar™ Pre-designed Human tRF&tiRNA Primer Sets are part of the nrStar™ tRF&tiRNA PCR System, and can be used in combination with the rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor) (AS-FS-003) for tRF&tiRNA detection and quantification.
All the primers have been validated in numerous sample types, and guarantee the most accurate and specific performance when used with Arraystar SYBR® Green qPCR Master Mix. Each primer pair produces a single specific amplification product, as demonstrated by amplification and dissociation curves (Fig. 1).
From RNA to individual tRF&tiRNA quantification
Purified total RNA (with small RNAs retained) is quality checked.
The rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor) (AS-FS-003) ligates adaptors and synthesizes cDNA from pretreated tRF&tiRNA fragments.
Pre-designed primer pairs are combined with cDNA and Arraystar SYBR® Green qPCR Master Mix.
qPCR is run on a standard real-time PCR instrument.
Individual tRF&tiRNA levels are quantified from the amplification and dissociation curves.
Product specifications, primer lists, and manuals
Human and Mouse primer lists provide full annotation of each assay: tRF&tiRNA name, tRF sequence, source tRNA, and catalog number. Each primer pair is sold with 200 reactions.
Individual tRF&tiRNA quantification applications
Accurate detection and quantification of specific tRFs and tiRNAs of interest by qPCR.
Validate tRF/tiRNA biomarker candidates identified by sequencing in larger sample cohorts.
Primers validated in numerous sample types support quantification in biofluids.
Quantify specific tRFs/tiRNAs in cancer, cardiovascular, and metabolic disease studies.
Recommended RNA input for pre-designed tRF&tiRNA primer set projects
Common questions about rtStar™ Pre-designed tRF&tiRNA Primer Sets
Featured Client Publications in tRF&tiRNA Research
rtStar™ Pre-designed tRF&tiRNA Primer Sets deliver validated, specific qPCR quantification — request a quote or ask about the primer list for your tRF/tiRNA of interest.