The rtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit is designed for reverse transcribing tRNAs efficiently. It removes internal modifications of tRNAs to ensure the proceeding of reverse transcriptase during cDNA synthesis, delivering magnitudes of increase in tRNA detection sensitivity and quantification accuracy.
The key to accurate tRNA reverse transcription
tRNAs carry extensive internal post-transcriptional modifications that block reverse transcriptase during cDNA synthesis. The rtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit efficiently removes internal modifications of tRNAs, whereas regular cDNA synthesis methods cannot, ensuring efficient first-strand cDNA synthesis.
This kit is the recommended companion for the nrStar™ tRNA PCR Array (Cat# AS-NR-001) and rtStar™ Pre-designed tRNA Primer Sets, and delivers magnitudes of increase in tRNA detection sensitivity, quantification accuracy, and assay discriminating power with the treated compared with untreated samples.
tRNA pretreatment enzymatically removes the extensive internal post-transcriptional modifications that block reverse transcription during cDNA synthesis. The rtStar™ kit combines this pretreatment with first-strand cDNA synthesis, enabling efficient, accurate, and sensitive tRNA detection by qPCR and sequencing.
Standard catalog product
| Product Name | Catalog No. | Size | Price |
|---|---|---|---|
| rtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit | AS-FS-004 | 12 reactions |
Key advantages of the rtStar™ kit
Efficiently removes internal modifications of tRNAs to ensure the proceeding of reverse transcriptase during cDNA synthesis, whereas regular cDNA synthesis methods do not.
Magnitudes of increase in tRNA detection sensitivity and quantification accuracy with the treated compared with untreated samples.
Optimized for reverse transcribing tRNAs efficiently, overcoming the barriers that hinder traditional cDNA synthesis.
Recommended companion for the nrStar™ tRNA PCR Array and rtStar™ Pre-designed tRNA Primer Sets.
Convenient 12-reaction format for direct use in tRNA qPCR workflows.
Why tRNA pretreatment is essential
tRNAs are heavily modified small RNAs: extensive internal and terminal post-transcriptional modifications block reverse transcription and distort readout. These modification barriers prevent regular cDNA synthesis methods from efficiently reverse transcribing tRNAs, causing biased and inaccurate detection.
The rtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit removes internal modifications of tRNAs before first-strand cDNA synthesis, enabling efficient reverse transcription. When applied with the nrStar™ tRNA PCR Array and rtStar™ Pre-designed tRNA Primer Sets, it delivers magnitudes of increase in tRNA detection sensitivity and quantification accuracy.
From total RNA to tRNA cDNA in three steps
Purified total RNA containing small RNAs is used as input, with quality and quantity assessment.
Internal modifications of tRNAs are efficiently removed by the pretreatment step, clearing the barriers that block reverse transcriptase.
Pretreated tRNAs are reverse transcribed into first-strand cDNA, ready for qPCR with the nrStar™ tRNA PCR Array or rtStar™ Pre-designed tRNA Primer Sets.
Product specifications and documentation
The rtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit is a complete, ready-to-use system for efficient tRNA reverse transcription. It is optimized for use with the nrStar™ tRNA PCR Array and rtStar™ Pre-designed tRNA Primer Sets.
Where the rtStar™ kit is used
Recommended companion for the nrStar™ tRNA PCR Array (Cat# AS-NR-001) for accurate isoacceptor and isodecoder profiling.
Pairs with rtStar™ Pre-designed tRNA Primer Sets for individual mature tRNA quantification.
The same pretreatment principle underlies Arraystar tRNA-seq and tRNA modification sequencing services.
Accurate tRNA detection supports studies of codon usage, translation efficiency, and tRNA biology.
Recommended RNA input for rtStar™ kit projects
Common questions about the rtStar™ kit
Featured Client Publications Using rtStar™ tRNA Pretreatment
The rtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit removes tRNA modification barriers for efficient, accurate cDNA synthesis — request a quote today.