Downstream-of-gene (DoG) RNAs are RNA transcripts that arise downstream of ~10% of host genes and are continuous with their upstream RNAs [1]. DoGs are defined as having a minimal length of 5 kb beyond the polyadenylation signal (PAS) at transcription end site (TES), with maximal length up to 200 kb in mammalian cells [1]. Due […]
DoG-derived chimera RNAs A chimeraRNA is produced by Cis-Splicing between Adjacent Genes (cis-SAGe) when RNA polymerase II skips the stop signals, generating a read-through pre-transcript of two neighboring genes (Fig. 1). It is estimated that ~5% of the tandem gene pairs in the human genome can be transcribed into single precursor RNAs and eventually spliced into chimeric RNAs.
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The identification and quantification of RNA G-quadruplex (rG4) in vivo is an essential step to study rG4 in cell biology and human diseases. Next-generation sequencing (NGS)-based techniques, such as G4RP-seq [1], BG4 uvRIP-seq, and DMS-seq plus RT Stop profiling[2], have been employed to investigate in rG4 vivo landscapes across the transcriptome and to assess quantitative
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Cancer metabolism is one of the core hallmarks of cancer [1, 2]. Mainly driven by oncogenic signaling pathways and by amplified or alternatively spliced metabolic enzymes, the characteristic and profound metabolic alteration allows cancer cells to accommodate metabolic demands to sustain growth, proliferation, and survival in nutrient fluctuating environment [3, 4](Fig. 1). Enhanced uptake of
Introduction R-loops are three-stranded RNA:DNA loops when the nascently transcribed RNA anneals with the template DNA strand and displaces the non-template DNA as unpaired single strand. Besides nascent mRNAs, long non-coding RNAs (lncRNAs) may also create and form R-loops. Mounting evidences now support cells can harness R-loops to regulate gene expression in ways including epigenetic regulation,
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Arraystar offers both tRNA Modification Seq-m1A/m3C/m1G/m2,2G and Small RNA Modification Microarray for small RNA modification profiling. The comparison chart below can help you choose the most appropriate tool for your research purpose. tRNA Modification Seq-m1A/m3C/m1G/m2,2G Small RNA Modification Microarray For tRNA only For multiple small RNA classes including miRNAs, pre-miRNAs, tRNAs and tsRNAs(tRFs and tiRNAs). Single-base resolution of modification sites, important info for
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How to Choose the Appropriate Tool for Small RNA Modification Research? Molecular Mechanisms of Small RNA Modifications Small RNA Modifications: Integral to Functions and Diseases The Challenges and Solutions of Studying Modified Small RNAs Profiling small RNA modifications is a key step in studying the increasingly important epitranscriptomics of the small RNA classes. However, not
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How to Choose the Appropriate Tool for Small RNA Modification Research? Molecular Mechanisms of Small RNA Modifications Small RNA Modifications: Integral to Functions and Diseases The Challenges and Solutions of Studying Modified Small RNAs Small RNAs are modified by chemical groups that regulate the RNA activities or endow them with new functions. By various molecular
How to Choose the Appropriate Tool for Small RNA Modification Research? Molecular Mechanisms of Small RNA Modifications Small RNA Modifications: Integral to Functions and Diseases The Challenges and Solutions of Studying Modified Small RNAs Introduction Small RNAs, including microRNAs (miRNAs) and tRNA-derived small RNAs (tsRNAs), harbor a diversity of RNA modifications. RNA modifications such as
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Most m6A modification occurs in m6A motifs having a core ACA sequence, collectively referred to as m6ACA sites. For reliable collection of m6ACA sites, we have established a pipeline to discover all m6ACA sites that are quantifiable by array probes. Most m6ACA sites are consist of a single-ACA that can be profiled for m6A methylation