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In RNA-seq, RPKM (Reads Per Kilobase of transcript per Million reads mapped) was introduced to normalize for differences in both library size and gene length. However, the RPKM calculation assumes that the total RNA content is the same across all samples. As a result, the RPKM value for a specific transcript reflects its relative abundance

Long non-coding RNAs (LncRNAs) often express and function at low abundance, buried in other classes of abundant RNAs (Fig. 1A and 1B)[1]. There are serious limitations of RNA-seq for lncRNA profiling. Poor quantification of lncRNAs due to the low abundance and the lack of completeness in lncRNA annotation For mere detection of the presence of

Unlike well established protein coding genes, publically available long non-coding RNAs (lncRNAs) are often sparsely annotated, partial in scope and scattered in collection. For example, large proportions of reported “lncRNAs” are assembled from short sequencing reads and tend to be incomplete at 5’ or 3’ends. Very often, cDNA libraries are truncated at 5’-ends due to

The Arraystar LncRNA microarray package includes systematic and detailed long non-coding RNA (lncRNA) annotations, subclassification, and analyses to gain insight into the complex biological functions of the lncRNAs. LncRNAs with reported biological processes or associated with human diseases are researched, annotated and cross referenced. This rich source of information helps to unravel functional roles and

nrStar™ Functional LncRNA PCR Arrays have an outstanding long non-coding RNA (lncRNA) content selection leading the field of LncRNA research. The LncRNAs that have been associated with diseases including cancers, cardiovascular diseases and neurodegenerative diseases are carefully categorized and annotated in detail to facilitate the researchers’ study and biomarker validation. Best coverage  Well-known LncRNAs characterized for

Overview Long non-coding RNAs (LncRNAs) are a class of non-protein coding RNAs longer than 200 nt. LncRNAs can regulate gene expression by various mechanisms with biological functions in, for example, embryonic development [1], stem cell pluripotency, cell lineage specification, genomic imprinting, cardiac development [2], hematopoiesis, immunity [3], and endocrine systems [4]. Dysregulated expression of lncRNAs

Long non-coding RNAs (LncRNAs) and mRNAs share certain common properties that allow them to be profiled together. However, lncRNAs also have characteristics significantly different from mRNAs, which pose special challenges for their expression profiling, particularly in the choice of profiling technologies. Arraystar lncRNA Arrays are specially designed to meet these challengers, offering irreplaceable benefits over