Reviews

In RNA-seq, RPKM (Reads Per Kilobase of transcript per Million reads mapped) was introduced to normalize for differences in both library size and gene length. However, the RPKM calculation assumes that the total RNA content is the same across all samples. As a result, the RPKM value for a specific transcript reflects its relative abundance […]

Long non-coding RNAs (LncRNAs) often express and function at low abundance, buried in other classes of abundant RNAs (Fig. 1A and 1B)[1]. There are serious limitations of RNA-seq for lncRNA profiling. Poor quantification of lncRNAs due to the low abundance and the lack of completeness in lncRNA annotation For mere detection of the presence of

Unlike well established protein coding genes, publically available long non-coding RNAs (lncRNAs) are often sparsely annotated, partial in scope and scattered in collection. For example, large proportions of reported “lncRNAs” are assembled from short sequencing reads and tend to be incomplete at 5’ or 3’ends. Very often, cDNA libraries are truncated at 5’-ends due to

The Arraystar LncRNA microarray package includes systematic and detailed long non-coding RNA (lncRNA) annotations, subclassification, and analyses to gain insight into the complex biological functions of the lncRNAs. LncRNAs with reported biological processes or associated with human diseases are researched, annotated and cross referenced. This rich source of information helps to unravel functional roles and

nrStar™ Functional LncRNA PCR Arrays have an outstanding long non-coding RNA (lncRNA) content selection leading the field of LncRNA research. The LncRNAs that have been associated with diseases including cancers, cardiovascular diseases and neurodegenerative diseases are carefully categorized and annotated in detail to facilitate the researchers’ study and biomarker validation. Best coverage  Well-known LncRNAs characterized for

Overview Long non-coding RNAs (LncRNAs) are a class of non-protein coding RNAs longer than 200 nt. LncRNAs can regulate gene expression by various mechanisms with biological functions in, for example, embryonic development [1], stem cell pluripotency, cell lineage specification, genomic imprinting, cardiac development [2], hematopoiesis, immunity [3], and endocrine systems [4]. Dysregulated expression of lncRNAs

Long non-coding RNAs (LncRNAs) and mRNAs share certain common properties that allow them to be profiled together. However, lncRNAs also have characteristics significantly different from mRNAs, which pose special challenges for their expression profiling, particularly in the choice of profiling technologies. Arraystar lncRNA Arrays are specially designed to meet these challengers, offering irreplaceable benefits over

Unlike protein coding genes, publically available long non-coding RNAs (lncRNAs) are often scantily annotated, partial in scope and scattered in collection. Arraystar maintains high quality proprietary transcriptome and lncRNA databases to extensively collect lncRNAs through our lncRNA discovery pipelines, external data sources, and knowledge-based mining of scientific publications. Arraystar Human LncRNA Array V4.0 has a total of

Microarrays have been used for decades as the standard technology platform for gene expression profiling.  In recent years, Next Generation Sequencing (NGS), which measures gene expression levels by quantifying transcript read densities in the whole transcriptome, has become increasingly popular for gene expression studies. Long non-coding RNAs (LncRNAs) are non-protein coding transcripts longer than 200

LncPath™ Pathway Focused LncRNA Arrays Long non-coding RNAs (LncRNAs) are non-coding RNA transcripts longer than 200 nt. LncRNAs play important roles in a wide range of biological processes. Dysregulated LncRNAs are associated with many complex human diseases such as cancers, Alzheimer’s disease, and heart diseases. To facilitate the functional study of LncRNAs and the understanding