Arraystar Super-Enhancer LncRNA Arrays are the first commercially available solution for sensitive and accurate SE-lncRNA profiling. The SE-lncRNAs are compiled on the foundation of Arraystar Gold standard & Reliable long non-coding RNA (lncRNA) collections and authoritative super-enhancer databases. “Super lncRNAs”, a special class of SE-lncRNA, are also included in the array contents. To experimentally observe, […]
Super-enhancer lncRNAs have been demonstrated master regulatory functions in biology and diseases, such as myogenesis, cardiomyocyte differentiation, erythropoiesis, fibrosis in post-myocardial infarction, and cancers. The innovative Arraystar Super-enhancer LncRNA Arrays simultaneously profile the super-enhancer lncRNAs and their target genes. The detailed annotation, analysis and cross-references are provided to facilitate the in-depth study on SE-lncRNAs and
Detailed Annotation and Analysis of Super-Enhancer LncRNAs and Their Target Genes Read More »
Introduction In higher eukaryotic cells, multi-exon genes can produce transcript isoforms by alternative splicing, greatly increase the variations of the encoded proteins. Many alternatively spliced transcript isoforms play distinct roles in different tissues and developmental stages. The aberrant expression of these transcript isoforms can lead to phenotypical changes and associate with diseases [1]. Similarly, super-enhancer
Transcript-Specific Probes on the Super-Enhancer LncRNA Arrays Read More »
Super-enhancer lncRNAs are generally labile and short in half-lives. They can function at low copy numbers per cell to activate the target genes by cis-mechanism. For example, LncRNA-HOTTIP can activate the HOXA gene cluster with an average of less than one copy per cell. To accurately detect and quantify the transient, low level SE-lncRNAs, Arraystar
In RNA-seq, RPKM (Reads Per Kilobase of transcript per Million reads mapped) was introduced to normalize for differences in both library size and gene length. However, the RPKM calculation assumes that the total RNA content is the same across all samples. As a result, the RPKM value for a specific transcript reflects its relative abundance
Microarrays Are More Adept at Low Abundance RNA Profiling Read More »
Long non-coding RNAs (LncRNAs) often express and function at low abundance, buried in other classes of abundant RNAs (Fig. 1A and 1B)[1]. There are serious limitations of RNA-seq for lncRNA profiling. Poor quantification of lncRNAs due to the low abundance and the lack of completeness in lncRNA annotation For mere detection of the presence of
What Are the Limitations of RNA-seq for LncRNA Profiling? Read More »
Unlike well established protein coding genes, publically available long non-coding RNAs (lncRNAs) are often sparsely annotated, partial in scope and scattered in collection. For example, large proportions of reported “lncRNAs” are assembled from short sequencing reads and tend to be incomplete at 5’ or 3’ends. Very often, cDNA libraries are truncated at 5’-ends due to
Human V5.0-Comprehensive and Robust Collection of Full-Length LncRNAs Read More »
The Arraystar LncRNA microarray package includes systematic and detailed long non-coding RNA (lncRNA) annotations, subclassification, and analyses to gain insight into the complex biological functions of the lncRNAs. LncRNAs with reported biological processes or associated with human diseases are researched, annotated and cross referenced. This rich source of information helps to unravel functional roles and
Towards Systematic and Functional Annotation of LncRNAs Read More »
nrStar™ Functional LncRNA PCR Arrays have an outstanding long non-coding RNA (lncRNA) content selection leading the field of LncRNA research. The LncRNAs that have been associated with diseases including cancers, cardiovascular diseases and neurodegenerative diseases are carefully categorized and annotated in detail to facilitate the researchers’ study and biomarker validation. Best coverage Well-known LncRNAs characterized for
Best Coverage and Comprehensive Annotation for Functional LncRNA PCR Array Read More »
Overview Long non-coding RNAs (LncRNAs) are a class of non-protein coding RNAs longer than 200 nt. LncRNAs can regulate gene expression by various mechanisms with biological functions in, for example, embryonic development [1], stem cell pluripotency, cell lineage specification, genomic imprinting, cardiac development [2], hematopoiesis, immunity [3], and endocrine systems [4]. Dysregulated expression of lncRNAs
Functional LncRNA PCR Array: From the Unknown to Biological Significance Read More »