TRAC-seq and HAC-seq are designed for precise detection of individual RNA modifications at single-nucleotide resolution. By combining selective chemical/enzymatic treatment with next-generation sequencing, these methods convert specific RNA modifications into characteristic reverse transcription signatures, enabling accurate identification and site-specific mapping of target modifications across the transcriptome. Their high sensitivity and nucleotide-level precision make them powerful tools for investigating the functional roles and regulatory dynamics of RNA modifications in diverse biological and disease contexts.
With the wealth of information from Arraystar m7G TRAC-Seq/m3C HAC-Seq service, investigators can easily advance their next step research in this field of study.
Benefits of Arraystar m7G TRAC-Seq Service
- Single-nucleotide resolution:Precise mapping of m7G sites at nucleotide-level accuracy.
• Chemical specificity: Highly specific chemical reactions, instead of antibody affinity, to eliminate background from non-specific antibody binding and enable quantitative stoichiometry assessment.
• Superior tRNA coverage: Demethylation step using Arraystar rtStar™ tRNA Pretreatment Kit to overcome tRNA modification barriers that block reverse transcription, allowing efficient sequencing of heavily modified tRNAs
• Comprehensive analyses: Simultaneous identification of m7G sites, and discovery of modification-associated sequence motifs (e.g. RAGGU motif for m7G tRNAs)
Benefits of Arraystar m3C HAC-Seq Service
- Single-Nucleotide Resolution: Provides precise mapping of m3C sites at nucleotide-level accuracy
- Chemical Specificity: Relies on highly specific chemical reactions rather than antibody affinity, eliminating background from non-specific binding and enabling quantitative stoichiometry assessment
- Reliable site identification: eliminates false positives by integrating m3C-demethylated samples as controls for significant cleavage induction and rescue
- Comprehensive Analysis: Enables simultaneous identification of m3C sites and discovery of modification-associated sequence motifs