Reviews

The Challenges and Solutions of Small RNA Profiling The Biases of Using TPM in Small RNA Sequencing Data Analysis Simultaneously Profile Multiple Small RNA Classes Accurately Direct End-labeling to Avoid the Biases From Sequencing Library Prep The comparison of small RNA transcript levels between samples using TPM in small RNA-sequencing (small RNA-seq) is actually meaningless. In small […]

The Challenges and Solutions of Small RNA Profiling The Biases of Using TPM in Small RNA Sequencing Data Analysis Simultaneously Profile Multiple Small RNA Classes Accurately Direct End-labeling to Avoid the Biases From Sequencing Library Prep To fluorescently label small RNAs for microarray profiling, the total RNA is treated with T4 polynucleotide kinase (T4 PNK) to remove

The Challenges and Solutions of Small RNA Profiling The Biases of Using TPM in Small RNA Sequencing Data Analysis Simultaneously Profile Multiple Small RNA Classes Accurately Direct End-labeling to Avoid the Biases From Sequencing Library Prep Mature small RNAs like miRNAs and tsRNAs have non-random, precisely cleaved termini and defined RNA sizes during biogenesis. Arraystar Small RNA

The Challenges and Solutions of Small RNA Profiling The Biases of Using TPM in Small RNA Sequencing Data Analysis Simultaneously Profile Multiple Small RNA Classes Accurately Direct End-labeling to Avoid the Biases From Sequencing Library Prep Small RNA-seq has been used for profiling microRNAs and other small RNAs. However, the use of small RNA-seq data to quantify