Mass Spectrometry (LC-MS)
LC-MS Based mRNA Modification Analysis Service
Arraystar LC-MS mRNA Modification Analysis Service analyzes 55 nucleoside modifications and characterizes global modification profile of mRNAs as mRNA biochemical properties vital to mRNA biogenesis, structure, functioning and implication in diseases.
Benefits
• Quantitative analysis of complex mRNA modifications from total RNA samples.
• Full service sample-to-data – From sample QC, mRNA enrichment, nucleoside analyte preparation, LC-MS/MS data acquisition, analysis to report.
• High Performance – Highly optimized experimental procedures, state-of-the-art LC-MS system, expertise in operation.
• Best analytical coverage – Simultaneous profiling of 55 nucleoside modifications in mRNAs.
Service Order Guide
1. Check if your modifications of interest are in our standard detection list.
2. If yes, please click “Request Quote” below, and our technical sales team will follow up shortly.
| Service Name | Catalog No | Size | Price |
|---|---|---|---|
| LC-MS Based mRNA Modification Analysis Service - Eukaryotic | AS-LC-m-S | 1 sample | |
| Total RNA Extraction Service | AS-RE-S | 1 sample |
Table1. Nucleoside modifications profiled by Arraystar LC-MS mRNA Modification Analysis
| Number | Nucleoside | Symbol | Number | Nucleoside | Symbol |
| 1 | 3′-O-methyladenosine | 3′-OMeA | 2 | 3′-O-methyluridine | 3′-OMeU |
| 3 | 2′-O-methylcytidine | Cm | 4 | 5-methyl-2-thiouridine | m5s2U |
| 5 | 3-methylcytidine | m3C | 6 | 5-methoxyuridine | mo5U |
| 7 | 5-methylcytidine | m5C | 8 | pseudouridine |  |
| 9 | N6-isopentenyladenosine | i6A | 10 | 2′-O-methylinosine | Im |
| 11 | 5,2′-O-dimethylcytidine | m5Cm | 12 | 3-methyluridine | m3U |
| 13 | 1-methyladenosine | m1A | 14 | 1-methylpseudouridine | m1 |
| 15 | 2-thiocytidine | s2C | 16 | 5-hydroxymethylcytidine | hm5C |
| 17 | N2, N2, 7-trimethylguanosine | m2,2,7G | 18 | 5,2′-O-dimethyluridine | m5Um |
| 19 | N4-acetyl-2′-O-methylcytidine | ac4Cm | 20 | N6-threonylcarbamoyladenosine | t6A |
| 21 | N6-methyladenosine | m6A | 22 | 2-methylthio-N6-threonylcarbamoyladenosine | ms2t6A |
| 23 | 3′-O-methylcytidine | 3′-OmeC | 24 | 5-carboxymethyluridine | cm5U |
| 25 | 2′-O-methyladenosine | Am | 26 | 5-methoxycarbonylmethyl-2-thiouridine | mcm5s2U |
| 27 | N2, N2-dimethylguanosine | m22G | 28 | 5-Methoxycarbonylmethyluridine | mcm5U |
| 29 | 5′-O-methylthymidine | 5′-OMeT | 30 | 2-methylthio-N6-isopentenyladenosine | ms2i6A |
| 31 | 2′-O-methyluridine | Um | 32 | Peroxywybutosine | o2w |
| 33 | inosine | I | 34 | 5-taurinomethyl-2-thiouridine | tm5s2U |
| 35 | 2′-O-methylguanosine | Gm | 36 | 5-oxyacetic acid uridine | cmo5U |
| 37 | 1-methylguanosine | m1G | 38 | 5-carbamoylmethyuridine | ncm5U |
| 39 | 7-methylguanosine | m7G | 40 | Queuosine | Q |
| 41 | N2-methylguanosin | m2G | 42 | 5-taurinomethyluridine | tm5U |
| 43 | 3′-O-methylinosine | 3′-OMeI | 44 | 5-formyl-2′-O-methylcytidine | f5Cm |
| 45 | 2-thiouridine | s2U | 46 | dihydrouridine | D |
| 47 | 4-thiouridine | s4U | 48 | 5-formylcytidine | f5c |
| 49 | 5-methyluridine | m5U | 50 | wybutosine | W |
| 51 | N4-acetylcytidine | ac4C | 52 | 5-methoxycarbonylmethyl-2′-o-methyluridine | mcm5Um |
| 53 | N6, O2′-methyladenosine | m6Am | 54 | 5-methylaminomethyl-2-thiouridine | mnm5s2U |
| 55 | 5-hydroxyuridine | ho5U |
Deciphering the interplay between SETD2 mediated H3K36me3 and RNA N6-methyladenosine in clear cell renal cell carcinoma (ccRCC). Shafiq Shaikh, et al. Epigenetics, 2025
Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells. Datta C,et al. Science Advances, 2022