N7-methylguanosine (m7G) is a pivotal tRNA modification in maintaining or enhancing tRNA structural integrity, stability, and translation efficiency of codon-enriched mRNAs. It functionally regulates stem cell self-renewal and development[1]. Its dysregulation drives tumorigenesis in diverse malignancies including leukemia, glioblastoma, cholangiocarcinoma, and lung cancer [2-4].

Arraystar m7G TRAC-Seq service offers unbiased, single-nucleotide resolution profiling of m7G modifications throughout the tRNA transcriptome. The detailed bioinformatics analyses facilitate insights into m7G in tRNA biology, diseases, and biomarker applications.

Benefits

• Single-nucleotide resolution: Precise mapping of m7G sites at nucleotide-level accuracy.
• Chemical specificity: Highly specific chemical reactions to eliminate background from non-specific antibody binding and enable quantitative stoichiometry assessment.
• Superior tRNA coverage: Demethylation step included to overcome tRNA modification barriers that block reverse transcription, allowing efficient sequencing of heavily modified tRNAs.
• Comprehensive analyses: DSimultaneous identification of m7G sites, and discovery of modification-associated sequence motifs (e.g. RAGGU motif for m7G tRNAs) (Fig. 1).

Fig 1. Comprehensive analyses. Left: m7G TRAC-seq read alignments to visualize m7G sites. Right: Motif analysis of m7G sites.

Arraystar tRNA NGS Solutions
From tRNA Expression, Modifications to Disease Mechanisms

• tRNA Sequencing

• tRNA Modification Sequencing

• m7G TRAC-Sequencing

• LncRNA Array
  [PMID: 41519884] Hyeon-Ji Kim,
  et al (2026) Signal Transduction and
  Targeted Therapy

• tRF&tiRNA Pretreatment Kit
   [PMID: 41555020] Xu M, et al.
  (2026) Nature Biotechnology

• tRF&tiRNA Sequencing
   [PMID: 41330933]
    FJunichiro Ishikawa, et al.
   (2026) Nat Communications

• Epitranscriptomic Array
   [PMID: 41717060] Hualong Zhu,et al.
   (2026) Eco-Environment & Health

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