Arraystar tRNA Modification Seq - m1A/m3C/m1G/m2,2G is designed to simultaneously and quantitatively profile tRNAs modified with multiple methylations (m1A, m3C, m1G, m2,2G) at single-base resolution. The modification is detected by methylation induced mutation index (MI) at each base position compared with its demethylase-treated tRNA. Among m1A, m3C, m1G, and m2,2G modifications, the m1G and m2,2G are further distinguished by their known distinct base positions in mammalian tRNAs (m1G at 9 and 37; m2,2G at 26 tRNA base positions).

Benefits:

• Multiple methylation modification detection.
Simultaneously profile m1A, m3C, m1G and m2,2G modifications in tRNAs

• High efficiency methylation removal. Efficiently remove internal and terminal modifications in the demethylase-treated tRNA.

• Single-base resolution. Identify the exact site of m1A, m3C, m1G and m2,2G methylation modifications by comparing demethylase-untreated tRNA with demethylase-treated tRNA side by side (Fig. 1).

• Comprehensive tRNA Epitranscriptomic Analysis
Analyze modifications sites, methylation levels, and tRNA expression all at once.

• Integrated tRNA Solutions. Work well together with our broad portfolio of tRNA research technologies:tRNA PCR array,LC-MS based tRNA modification analysis,tRF&tiRNA-seq, and tRF&tiRNA PCR array.

Figure 1. Single-base resolution by tRNA Modification Seq.

• Epitranscriptomic Array
   [PMID: 38355624] Xiong YW, et al.
   (2024) Nature Communications

• Small RNA Modification Array
   [PMID: 38747146] Zhang J, et al.
   (2024) Circulation Research

• Circular RNA Array
   [PMID: 39695693] Hu Y, et al.
   (2024) Molecular Cancer

• tRNA Sequencing
   [PMID: 38519786] Wu X, et al.
   (2024) Nature Cancer

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- Circular junction-specific probes
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Valid 06/01/2025 - 08/31/2025