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Customer Success Story
tRNA derived small RNAs (tsRNAs) are a new category of non-coding small RNAs with regulatory functions. By using Arraystar tsRNA-seq for profiling, Small RNA Modification Array for epitranscriptomic study, and LC/MS for RNA modification analysis, a novel m7G-modified tsRNA, m7G-3’tiRNA-LysTTT (mtiRL), was discovered in chemical carcinogenesis models[1]. mtiRL, modified by m7G-writer enzyme mettl1, promotes bladder cancer (BC) malignancy in vitro and in vivo. Mechanistically, mtiRL binds oncoprotein Annexin A2 (ANXA2) to promote its Tyr24 phosphorylation via enhanced interaction between ANXA2 and Yes1 proto-oncogene, leading to ANXA2 activation and nuclear localization. The findings define a critical role for mtiRL and suggest a potential new way for cancer treatment.
Reference
[1] Ying X. et al (2024) “A Novel tsRNA, m(7)G-3' tiRNA Lys(TTT), Promotes Bladder Cancer Malignancy Via Regulating ANXA2 Phosphorylation”
Adv Sci (Weinh) 11(31):e2400115 [PMID:38894581]
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Array tRF&tiRNA Sequencing Service
• Performance optimized tRFs&tiRNAs sequencing method with rigorous QC process
• Precise annotation and classification system based on tRNA topology and statistical significance.
• Additional miRNA expression and differential analyses
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Arraystar Small RNA Modification Array Service
• Coverage of multiple small RNA classes, including miRNAs, pre-miRNAs, and tsRNAs (tRFs and tiRNAs)
• Direct RNA end labeling ensures high fidelity of quantification, without the problem of modification blocked cDNA synthesis during RNA-seq library prep.
• High sensitivity for modified small RNAs at lower levels
• Low sample amount required, starting from as little as 2 µg total RNA.
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