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Customer Success Story
Featured in the front cover of Nature Structural & Molecular Biology, the translatome of translational control of brain development has been established, where Arraystar tRNA qPCR array was used to profile tRNA isodecoders across five stages of mouse neocortex neurogenesis [1]. tRNA abundance was analyzed for ribosome occupancy, usage availability, and codon optimality. At codon resolution, a critical period of translational control during brain development is discovered. tRNA is a strong determinant of ribosome occupancy.
Reference
[1] Harnett D. et al (2022) A critical period of translational control during brain development at codon resolution.
Nat Struct Mol Biol 29(12):1277-1290 [PMID:36482253]
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Arraystar nrStar™ tRNA PCR Arrays
• Profile all the nuclear tRNA isoacceptors catalogued in GtRNAdb
• Profile all tRNA isodecoders distinguishable by qPCR
• Focus on the high confidence tRNAs (tRNAScan score >50)
• Experimentally validated primers across numerous tissues and cell lines
• Easy-to-use convenient panel to obtain results in hours
• Work best with rtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit that efficiently removes tRNA methylations to achieve a new level of detection accuracy.
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