To profile the full spectrum of small RNA modifications with high sensitivity and accurate stoichiometry, Arraystar has developed the Small RNA Modification Profiling Technology (Fig 1), where it integrates microarray with RNA modification immunoprecipitation to simultaneously measure the levels of modified and unmodified small RNAs, providing key quantitative information to study the regulatory function and biology of the small RNAs.

• Capable of detecting and quantifying a variety of small RNA modifications, e.g. o8G, m7G, m6A, Ψ, or m5C.
• Coverage of multiple small RNA classes, including miRNAs, pre-miRNAs, and tsRNAs (tRFs and tiRNAs)
• Gold standard for accurate quantification of modified small RNAs
• High sensitivity for modified small RNAs at lower levels
• Low sample amount requirements, starting from as little as 1 ug total RNA.

Fig 1. Arraystar Small RNA Modification Profiling Technology. Modified small RNAs are immunoprecipitated by a specific antibody, and then identified and quantified by Arraystar small RNA modification microarray.