ChIP-chip combines Chromatin Immunoprecipitation (ChIP) with microarray technology to analyze how regulatory proteins interact with the genome and provide insight into the mechanisms for histone modification and the regulation of transcription factors.
The promoter arrays that are most often selected by our customers include:
|
Microarray |
Species |
Array Format |
Coverage |
|
Multiplex HG18 Refseq Promoter |
Human |
3 * 720K |
22,542 promoters: -3.2kb ~ +0.8kb (30,893 transcripts) |
|
Multiplex MM9 Refseq Promoter |
Mouse |
3 * 720K |
20,404promoters: -3.2kb ~ +0.8kb (22,881transcripts) |
|
Multiplex RN34 Refseq Promoter |
Rat |
3 * 720K |
15,287 promoters: -4.28kb ~ +1.07kb (15600 transcripts) |
|
MM8 Deluxe Promoter HX1 |
Mouse |
1 * 2.1M |
20,000 promoters: -8.2kb ~ +3kb
249 miRNA promoters ( -20kb~ +1kb) |
Description of Services
Please refer to Sample Submission for details in how to get your project started.
Customer to provide: Chromatin immunoprecipitated DNA enriched by specific antibodies
Arraystar to provide:
� Quality assessment of ChIP
� Linear amplification using SIGMA WGA kit
� Target preparation by labeling with Cy3/5
� Array hybridization, washing, and scanning
� Data extraction, analysis and summarization
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